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Nerve cells derived from human stem cells often serve as the basis for research into brain diseases. However, these cells differ considerably in their quality and produce varying results. Scientists around the world are therefore looking for simple cell models that lead to consistent results when an experiment is repeated. It was obtained from pluripotent stem cells through a fast forward programming method and provides highly standardized conditions for investigating nerve cell functions. The two studies have now been published in the international journal Cell Reports. Using cell reprogramming, so-called induced pluripotent stem cells iPS cells can be generated from a blood or skin sample.

A nerve cell serves as a "single" for studies

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Synaptic dysfunction is associated with many brain disorders, but robust human cell models to study synaptic transmission and plasticity are lacking. Instead, current in vitro studies on human neurons typically rely on spontaneous synaptic events as a proxy for synapse function. Here, we describe a standardized in vitro approach using human neurons cultured individually on glia microdot arrays that allow single-cell analysis of synapse formation and function.

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